3' Primer
Neo-R: GCCCAGTCATAGCCGAATAG
5' Primer
SV40pro-F: TATTTATGCAGAGGCCGAGG
Cloning Method
MCS, Restriction endonucleases, Hind III
Cloned Strain(s)
DH5α, HB101 etc.
Host cells (lines)
Conventional cell lines, 293, CV-1, CHO, etc.
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Mammalian cell vectors