3' Primer
SV40pA rev: GAAATTTGTGATGCTATTGC
5' Primer
CMV fwd: CGCAAATGGGCGGTAGGCGTG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α etc.
Host cells (lines)
Mammalian cells
Stable
Transient expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Tag
IgK, HA tag (N-terminal), 2XMyc tag (N-terminal)
Plasmid Type
Mammalian expression vectors, Capture-Tec systems, Immunomagnetic beads