Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
TOP10, DH5α, JM109
Host cells (lines)
Conventional cell lines, 293, CV-1, CHO, etc.
Stable
Transient or stable expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Mammalian cell expression vectors