3' Primer
pcDNA3.4-R: CAACATAGTTAAGAATACCAGTC
5' Primer
CMV-F: CGCAAATGGGCGGTAGGCGTG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α etc.
Host cells (lines)
Conventional cell lines, 293, CV-1, CHO, etc.
Stable
Transient expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Mammalian cell vectors, Transient expression vectors