3' Primer
pBABE 3': ACCCTAACTGACACACATTCC
5' Primer
CMV-F: CGCAAATGGGCGGTAGGCGTG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α, TOP10 etc.
Host cells (lines)
293, CHO etc.
Stable
Transient expression
Selectable Marker
Neomycin
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Mammalian cell expression vectors