Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α, TOP10
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Tag
His Tag (N-terminal), Xpress tag (N-terminal); V5 tag (C-terminal), His tag (C-terminal)
Plasmid Type
Saccharomyces cerevisiae expression vectors, Nutritionally deficient screening vectors, Centromeric plasmids