3' Primer
GFP-R primer: ACCATCTAATTCAACAAGAATTGGGACAAC
5' Primer
M13/pUC fwd primer: CCAGTCACGACGTTGTAAAACG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH10B competent cells, 30°C
Host cells (lines)
Sprouting yeast (Saccharomyces cerevisiae), Candida albicans
Stable
Transient expression
Selectable Marker
URA3, Nourseothricin
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Saccharomyces cerevisiae expression vectors, Integrated vectors, FLP/FRT system vectors
Datasheet 
