3' Primer
M13 rev primer: GGAAACAGCTATGACCATG
5' Primer
M13/pUC fwd primer: CCAGTCACGACGTTGTAAAACG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH10B competent cells, 30°C
Host cells (lines)
Sprouting yeast (Saccharomyces cerevisiae), Candida albicans
Stable
Transient expression
Selectable Marker
Nourseothricin
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Tag
3XMyc tag (C-terminal)
Plasmid Type
Saccharomyces cerevisiae vectors, Replicating vectors, Resistance screening vectors
Datasheet 
