Iron Oxidizing Medium is used for the isolation, cultivation and enrichment of Thiobacillus ferroxidans.
Form
White to cream homogeneous free flowing powder
Characteristic
Cultural characteristics observed after an incubation at 30 centigrade upto 5 days. Reaction of (0.39 gm Part A in70 ml distilled water containing 1 ml of 10N sulphuric acid)+ 2.42 gm of Part B in 30 ml distilled water at 25 centigrade. pH : 3.3±0.3.
Storage
Store below 30 centigrade in tightly closed container and prepared medium at 2-8 centigrade. Use before expiry period on the label.
Instructions
Suspend 3.85 grams (the equivalent weight of dehydrated medium per litre) of Part A in 700 ml distilled water containing 1 ml of 10N sulphuric acid. Heat to boiling to dissolve the medium completely. Suspend 24.16 grams (the equivalent weight of dehydrated medium per litre) of Part B separately in 300 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize Part A and Part B separately by autoclaving at 15 lbs pressure (121 centigrade) for 15 minutes. Cool each solution to 25 centigrade. Aseptically add 300 ml of sterilized Part B to 700 ml of Part A. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
For research or industrial raw materials, not for personal medical use!
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