3' Primer
GCAAATGGCATTCTGACATCC
5' Primer
GACTGGTTCCAATTGACAAGC
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
TOP10, JM109etc.
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Tag
V5 Epitope Tag (C-terminal), His Tag (C-terminal)
Plasmid Type
Saccharomyces cerevisiae expression vectors, Nutritionally deficient screening vectors