3' Primer
CYC1-R: GCGTGAATGTAAGCGTGAC
5' Primer
GAL1-F: AATATACCTCTATACTTTAACGTC
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
TOP10, E.coli
Constitutive/Inducible
Inducible
Induction Method
Galactose
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Saccharomyces cerevisiae expression vectors