3' Primer
3´ AOX1: GCAAATGGCATTCTGACATCC
5' Primer
5´ AOX1: GACTGGTTCCAATTGACAAGC; alpha-Factor-F: TACTATTGCCAGCATTGCTGC
Cloning Method
MCS, Restriction endonucleases
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin and Kanamycin
Tag
N-alpha factor, C-His
Plasmid Type
Pichia pastor protein expression vectors