Product Name
ISP Medium No. 3
Product Overview
ISP Medium No. 3 is used for the cultivation and characterization of Streptomyces species.
Form
Cream to beige homogeneous free flowing powder
Characteristic
Cultural characteristics observed after an incubation at 35-37 centigrade for 48-72 hours.
Reaction of 3.8% w/v aqueous solution at 25 centigrade. pH : 7.3±0.2.
Storage
Store below 30 centigrade in tightly closed container and prepared medium at 2-8 centigrade. Use before expiry period on the label.
Instructions
Suspend 38 grams(equivalent weight of dehydrated medium per litre) in 1000 ml distilled water. Heat to boiling to dissolvethe medium completely. Sterilize by autoclaving at 15 lbs pressure (121 centigrade) for 15 minutes. Cool to 45-50 centigrade and mixintermittently while pouring into sterile Petri plates for even distribution of oatmeal.