Product Name
ISP Medium No. 4 (Inorganic Salt Starch Agar)
Product Overview
ISP medium No. 4 (Inorganic Salt Starch Agar) is used for cultivation and characterization of Streptomyces species.
Form
Cream to yellow homogeneous free flowing powder
Characteristic
Cultural characteristics observed after an incubation at 30-32 centigrade for 48-72 hours.
Reaction of 3.65% w/v aqueous solution at 25 centigrade. pH : 7.2±0.2.
Storage
Store below 30 centigrade in tightly closed container and prepared medium at 2-8 centigrade. Use before expiry period on the label
Instructions
Suspend 36.5 grams of dehydrated medium in 1000 ml distilled water. Heat just to boiling. Sterilize by autoclaving at 15 lbspressure (121 centigrade) for 15 minutes. Agitate constantly while pouring into sterile Petri plates to obtain a uniform suspension.