Replicator
pUC ori, F1 ori, 2 mu ori
Terminator
CYC1 terminator
3' Primer
CYC1-R: GGGACCTAGACTTCAGGTTG
5' Primer
CYC1-F:CTTTCCTTATACATTAGGACC
Cloned Strain(s)
DH5α etc.
Host cells (lines)
Yeast cells
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Yeast cell plasmids, Yeast hybridization plasmids, Two-hybrid plasmids