3' Primer
GFP-R primer: TAGTTTTCCAGTAGTGCAAATAAATTTAA
5' Primer
KS primer: TCGAGGTCGACGGTATC
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH10B competent cells, 30°C
Host cells (lines)
Sprouting yeast (Saccharomyces cerevisiae), Candida albicans
Stable
Transient expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Saccharomyces cerevisiae vectors, Overexpression vectors, Recipient plasmids
Datasheet 
