3' Primer
GCAAATGGCATTCTGACATCC
5' Primer
GACTGGTTCCAATTGACAAGC
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α, TOP10, JM109
Constitutive/Inducible
Inducible
Induction Method
Methanol
Bacterial Resistance(s)
Blasticidin
Tag
c-Myc Epitope Tag, His Tag (6x)
Plasmid Type
Pichia pastor expression vectors, Secretory expression vectors, Extracellular expression vectors