5' Primer
CMV fwd: CGCAAATGGGCGGTAGGCGTG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α or HB101
Host cells (lines)
Packaged cell lines such as 293T
Stable
Transient expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Mammalian vectors, Retroviral packaging, Single plasmid packaging systems