3' Primer
ACCTACAGGTGGGGTCTTTCATTCCC
5' Primer
AGCCCTCACTCCTTCTCTAG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α etc.
Host cells (lines)
Conventional cell lines, 293, CV-1, CHO, etc.
Stable
Transient or stable expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Viral/Non-Viral
Retrovirus
Plasmid Type
Retroviral vectors, Library cloning and expression vectors