Promoter
native cas9 promoter
3' Primer
AraC-F-New: GATGTAGCCGTCAAGTTGTCA
5' Primer
pENTR1A-3: GTAACATCAGAGATTTTGAGACAC
Cloning Method
MCS, Restriction endonucleases
Host cells (lines)
Bacteria
Constitutive/Inducible
Inducible
Bacterial Resistance(s)
Kanamycin
Tag
NLS、His8 (C-terminal)
Plasmid Type
CRISPR/Cas9 vectors, Knockout vectors, Prokaryotic vectors