Product Name
PM Cmm Agar Base
Product Overview
Phytopathogenic Microbe Cmm agar base is semi-selective medium for the detection of Clavibacter michiganesis subsp michiganesis on seeds of tomato.
Form
Light yellow coloured, homogeneous, free flowing powder
Characteristic
Cultural characteristics observed with added CNNP supplement (PHS004), after an incubation at 30-32 centigrade for 5-6 days .
Reaction of 3.22% w/v aqueous solution is pH 7.3 at 25 centigrade.
Storage
Store below 30 centigrade and the prepared medium at 2 - 8 centigrade.
Instructions
Suspend 32.2 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize the medium by autoclaving at 15 lbs pressure (121 centigrade) for 15 minutes. Cool to 45-50 centigrade and aseptically add the rehydrated contents of one vial of CNNP supplement. Mix well and pour into sterile Petri plates.