PM Tbb Agar Base

AROM-810

PM Tbb Agar Base

Phytopathogenic Microbe Tbb agar base is semi-selective medium for the detection of Xanthomonas campestris pv.vesicatoria and Xanthomonas vesicatoria on seeds of pepper and tomato.

Cultural characteristics observed with added CCFTNB supplement-1, after an incubation at 30-32 centigrade for 5-6 days .
Reaction of 3.22% w/v aqueous solution is pH 6.6 at 25 centigrade.

Store below 30 centigrade and the prepared medium at 2 - 8 centigrade.

Suspend 32.2 grams in 1000 ml distilled water containing 10 ml Tween 80. Heat to boiling to dissolve the medium completely. Sterilize the medium by autoclaving at 15 lbs pressure (121 centigrade) for 15 minutes. Cool to 45-50 centigrade and aseptically add the rehydrated contents of one vial of CCFTNB supplement-1 Mix well and pour into sterile Petri plate.