3' Primer
T7 terminator: GCTAGTTATTGCTCAGCGG
5' Primer
T7 Forward: TAATACGACTCACTATAGGG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α etc.
Host cells (lines)
Many genera
Constitutive/Inducible
Inducible
Bacterial Resistance(s)
Streptomycin
Tag
T7 tag (N-terminal), 6xHis tag (C-terminal)
Plasmid Type
Broad host vectors, Bacterial expression vectors, In vitro expression vectors