3' Primer
M13 Reverse: CAGGAAACAGCTATGAC
5' Primer
M13 Forward: TGTAAAACGACGGCCAGT
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α etc.
Host cells (lines)
Many genera
Constitutive/Inducible
Inducible
Bacterial Resistance(s)
Streptomycin
Plasmid Type
Broad host vectors, Cloning vectors, Prokaryotic expression vectors