Product Name
Potato Dextrose Broth
Product Overview
Potato Dextrose Broth is recommended for the isolation and enumeration of yeasts and moulds.
Form
Off-white to yellow homogeneous free flowing powder
Characteristic
Cultural characteristics observed after an incubation at 25-30 centigrade for 4-5 days.
Reaction of 2.4% w/v aqueous solution at 25 centigrade. pH : 5.1±0.2.
Storage
Store below 30 centigrade in tightly closed container and the prepared medium at 2 - 8 centigrade.
Instructions
Suspend 24 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 centigrade) for 15 minutes. Mix well before dispensing. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml of sterile, cooled medium is approximately 1 ml. Do not heat the medium after addition of the acid.