5' Primer
AGCTGGACATCACCTCCCACAACG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
TOP10, DH5α, HB101
Host cells (lines)
Both prokaryotic and eukaryotic cells
Stable
Transient or stable expression
Selectable Marker
Neomycin
Bacterial Resistance(s)
Kanamycin
Plasmid Type
Promoterless vectors, Fluorescent reporting vectors