5' Primer
LacI-R: GGCATACTCTGCGACATCGT
Tac promoter:Â GAGCGGATAACAATTTCACACAGG
Cloning Method
MCS, Restriction endonucleases
Constitutive/Inducible
Inducible
Bacterial Resistance(s)
Chloramphenicol
Plasmid Type
Corynebacterium glutamicum/E. coli shuttle plasmids