3' Primer
M13-R: CAGGAAACAGCTATGAC
5' Primer
M13 (-21) Forward: TGTAAAACGACGGCCAGT or M13 (-40) Forword: GTTTTCCCAGTCACGAC
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α etc.
Host cells (lines)
Streptomyces, Actinomyces, etc.
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Apramycin
Plasmid Type
Streptomyces integrative vectors, Streptomyces gene overexpression vectors