Instructions
Rehydrate the contents of one vial aseptically with 10 ml of 50% acetone. Mix well, avoiding frothing to dissolve and aseptically add it to 990 ml of sterile, molten, cooled (45-50 centigrade) SM Selective Agar Base alongwith 1 vial of TTC Solution 1%. Mix well and pour into sterile petri plates.