MAV203 Yeast Competent Cells

MAV203 Yeast Competent Cells

Size Price Qty Online Order
100 μl*50 $738 Order Online  Add to Quote

Handling and shipping fee may apply.

Cat#
YCC-013
Product Name
MAV203 Yeast Competent Cells
Size
100 μl*50
Description
MAV203 strain, MATα type, can be directly transformed for plasmid manipulation for protein interaction validation or screen library assays.
Genotypes
MATα; leu2-3,112; trp-901; his3Δ200; ade2-101; cyh2R; can1R ; gal4Δ; gal8Δ; GAL1::lacZ; HIS3UASGAL1::HIS3@LYS2; SPAL
Reporter Genes
Ura, HIS, Trp, Leu
Transformation Efficiency
Transformation efficiency >104 cfu/ug DNA as assayed by pGADT7 plasmid.
Usage Instructions
1). Take a sterile 1.5 ml EP tube, sequentially add 1-3 μg of pre-cooled target plasmid, 10 μl of Carrier DNA (95-100 ℃ for 5 min in a rapid ice bath, repeat once), and 100 μl of competent cells melted on ice, 500 μl of PEG/LiAc, and gently turn and mix 6-8 times.
2). 30 ℃ water bath for 30 min, every 10 min gently turn over and mix 6-8 times.
3). Add 20 μl of dimethyl sulfoxide to each branch (used to improve the conversion efficiency, can not be done).
4). 42 ℃ water bath for 15 min, every 5 min gently turn over and mix 6-8 times.
5).12,000 rpm instantaneous centrifugation and discard the supernatant, add 1 ml of YPD Plus to each cartridge, and resuscitate at 30℃ for 1 h (used to improve the transformation efficiency, can not be done).
6).12,000 rpm instantaneous centrifugation and discard the supernatant, resuspend with 100 μl of 0.9% NaCl, coat the plate, and incubate at 30℃ for 48-96 hours.
Notes
Competent cells are best melted on ice, PEG solution will precipitate at low temperatures, please dissolve completely at room temperature before use.
Size of Carrier DNA
5 μg/μl, 100 μl
Size of PEG/LiAc
5 ml
Shipping and Storage
Competent cells are transported on dry ice and stored at -80°C. Carrier DNA needs to be stored at -20°C. PEG/LiAc needs to be stored at 4°C.
Valid Period
6 months
Our products/services are For Research Use Only. Not For Clinical Use!
Online Inquiry
0
Inquiry Basket