Product Name
Soy GMO RR Kit (24)
Product Overview
This is a real-time PCR-based detection kit of Roundup Ready soy in food, animal feed, and pharmaceutical products.
Description
The Soy GMO RR Kit (24) is designed for the target-specific detection of Roundup Ready soy in food, animal feed, and pharmaceutical products. The assay uses a real-time PCR-based protocol.
Storage
GMO Detection Kit are shipped on dry ice. Multiplex PCR Master Mix and ROX dye should be stored immediately at –20°C upon receipt, in a constant-temperature freezer. All remaining not reconstituted kit components should be stored at 2–8°C and protected from light. Stored under these conditions and handled correctly, assay performance remains unaffected until the date of expiration printed on the quality control label inside the kit box or envelope. Reconstituted reagents of GMO Detection Kit should be dispensed into aliquots to avoid more than 5 freeze–thaw cycles, and stored at 2–8°C for short-term storage (1 month) or –20°C for long-term storage.
Kit Components
PCR Assay RR Soy;
Internal Control;
Positive Control;
Multiplex PCR Master Mix;
Nucleic Acid Dilution Buffer;
RNase-Free Water;
50x ROX Dye Solution
Scientific Background
The Soy GMO RR Kit (24) is designed for the qualitative detection of the genetically modified organism (GMO) Roundup Ready soy in food and animal feed samples. The genetic modification in the RR soy line provides resistance to the active compound glyphosate in herbicides, which is detected by the RR Soy Kit. RR soy is the most frequently used genetically modified soy line.
Detection of GMOs using real-time PCR is based on the amplification of a specific region of the relevant GMO genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.
Detection method
Real-time PCR
Features & Benefits
Highly sensitive and specific detection of Roundup Ready soy;
Streamlined detection protocol;