GMO Detection Kit (50)

GMO-118

GMO Detection Kit (50)

The GMO Detection Kit (50) is designed for identifying GMO in plant and seeds based on the detection of the Agrobacterium tumifaciens tNOS gene sequences by real-time PCR followed by MCA.

The GMO Test Kit (50) uses primers to amplify and detect part of the tNOS terminator sequence and a conserved region of the chloroplast gene as an extraction control by real-time PCR. Amplification of the control ensures that the extract has been prepared correctly and the PCR Master Mix functions correctly. The amplification plot does not differentiate between the tNos and Chloroplast gene products. Melt Curve Analysis (MCA) is used to further differentiate between the tNos and chloroplast target sequence based on melting temperature of the amplified products.

Spin down all tubes to collect contents at the bottom of the tube before opening the caps. It is recommended to keep reconstituted reagents on ice. Store all dry components at -20 °C for longterm storage. Once the various components are reconstituted, store at -20 °C and avoid repeated freeze-thaw.

Plant Extraction Buffer A 5 mL;
Enzyme B Lyophilized;
Reconstitution Buffer “C” 0.05 mL;
tNos DNA (control) Lyophilized;
Primers Lyophilized;
PCR Master Mix + Hot Start DRY Lyophilized;
Reconstitution Buffer 0.7 mL;
Green 20X Dye 1 mL;
Water 5 mL;
1.5 mL tubes 30;
8-strip PCR Strip Tubes 15;
8-strip PCR Strip Caps 15.

Thermocycler with capabilities for real-time PCR and melt curve analysis;
Low speed mini centrifuge for microfuge tubes and PCR tubes;
Vortexer;
Tube racks for PCR tubes and microfuge tubes.

Genetically modified organisms are either of plant or animal origin, and contain an altered characteristic or trait. We will focus on genetically modified plants. One of the most common genetic modifications of plants is to include a gene encoding a toxin from a bacterium that confers resistance to various insect pests. GMO plants/plant products are characterized by the presence of the cauliflower mosaic (CaMV) 35S promoter and the terminator sequence of the nopaline synthase (tNOS) gene from Agrobacterium tumifaciens used to control gene expression of the foreign gene.

Real-time PCR

Crude extract from leaves or frozen corn.