Bt Cry1Ab/Cry1Ac ELISA GMO Detection Kit

GMO-123

Bt Cry1Ab/Cry1Ac ELISA GMO Detection Kit

Bt Cry1Ab/Cry1Ac ELISA GMO Detection Kit is designed for the detection of Bt Cry1Ab/Cry1Ac protein in corn seed, cotton seed, leaf tissue, surface water, and soil samples.

The Bt Cry1Ab/1Ac ELISA is an immunoassay for the qualitative screening detection of Bt Cry1Ab/1Ac protein residues. The Bacillus thuringiensis (Bt) Cry1Ab/1Ac protein is an insecticidal crystalline protein expressed by the Cry1Ab/1Ac gene in certain strains of genetically modified plants. This Bt Cry1Ab/Cry1Ac ELISA Detection Kit is suitable for the qualitative detection of Bt Cry1Ab/1Ac in MON810, Bt11, Bollgard®, Bollgard II®, or WideStrike® corn seed, cotton seed, leaf tissue, surface water, and soil samples (please refer to the appropriate sample preparation or extraction). If necessary, positive samples can be confirmed by PCR or other conventional methods.

The Bt Cry1Ab/Cry1Ac ELISA GMO Detection Kit should to be stored in the refrigerator (4–8°C). The solutions must be allowed to reach room temperature (20-25°C) before use. Reagents may be used until the expiration date on the box.

The positive control solution in the test kit contain small amounts of Bt Cry1Ab/1Ac. In addition, the substrate solution contains tetramethylbenzidine (TMB) and the stop solution contains diluted sulfuric acid. Avoid contact of sub-strate and stopping solutions with skin and mucous membranes. If these reagents come in contact with the skin, wash with water.

Microtiter plate coated with an anti-Bt Cry1Ab/1Ac polyclonal antibody, in a re-sealable aluminum pouch, 96 wells;
Bt Cry1Ab/1Ac Positive Control Solution,1 mL;
Negative Control Solution, 1 mL;
Anti-Bt Cry1Ab/1Ac polyclonal antibody-HRP conjugate, 4 vials (lyophilized);
Antibody conjugate diluent, 12 mL;
Wash Buffer (5X) Concentrate, 100 mL, must be diluted before use, see Test Preparation (Section D);
Extraction Solution/Sample Diluent (5X) Concentrate, 2 x 25 mL, must be diluted before use, see Test Preparation (Section D); Substrate (Color) Solution (TMB), 12 mL;
Stop Solution, 6 mL (handle with care)

Micro-pipettes with disposable plastic tips (20-1000 μL);
Multi-channel pipette (50-250 μL) or stepper pipette with disposable plastic tips (50-250 μL);
Microtiter plate reader (wave length 450 nm);
Container with 500 mL capacity (for diluted 1X Wash Buffer);
Deionized or distilled water;
Timer;
5 mL microcentrifuge vials;
Analytical 3 place balance (optional);
Pipette bulbs;
Vortex mixer;
4 mL glass vials with Teflon-lined caps;
Centrifuge capable of spinning at 3,000 rpm (1500 x g) (optional);
15 mL or 50 mL centrifuge vials (optional);
Low protein binding syringe filter (0.8/0.2 μm) with syringe (optional);
Small plastic bags or wax paper;
Pliers, hammer, seed crusher with microcentrifuge tubes

Bt Cry1Ab/Cry1Ac, a protein derived from the bacteria Bacillus thuringiensis, is expressed in certain genetically modified (GM) corn and cotton plants, such as YieldGard® corn and Bollgard®, WideStrike® cotton. The Cry1Ab/Cry1Ac protein targets the larvae of multiple Lepidopteran (moth) species by binding to specific receptors in the larva’s gut with cascading effects that ultimately leads to death (1).
Routes of exposure affecting regulatory decisions include direct contact, such as non-target organisms feeding on crop tissues, seeds, or plant residues containing Cry1Ab/Cry1Ac, and indirect contact, such as exposure to the toxin in pollen, soil contaminated by decomposing plant material containing Cry1Ab/Cry1Ac, and predators consuming target species (2).
Although initial testing showed low enviromental impact and toxicity, controversy has arisen over the long-term impact of GM crops on the environment and whether or not GM foods are safe for consumption. Several animal studies have shown serious health risks, such as infertility, immune problems, accelerated aging, faulty insulin regulation, and changes in major organs including the gastrointestinal system, can be associated with the consumption GM products (3).
Bt proteins have been shown to leach into the soil and streams (4).
Efforts like the Non-GMO Project are raising awareness towards the growing number of problems associated with this technology and generat-ing a larger public demand for manufacturers to label their products accordingly.
The Bt Cry1Ab/Cry1Ac ELISA GMO Detection Kit allows for the analysis of 46 samples in duplicate determination. The test can be performed in 90 minutes.

ELISA Detection

Corn seed, cotton seed, leaf tissue, surface water, and soil samples