Bt Cry1F ELISA GMO Detection Kit

GMO-124

Bt Cry1F ELISA GMO Detection Kit

Bt Cry1F ELISA GMO Detection Kit is designed for the detection of Bt Cry1F protein in corn seed, cotton seed, leaf tissue, surface water, and soil samples.

The Bt Cry1F ELISA is an immunoassay for the qualitative screening detection of Bt Cry1F protein residues. The Bacillus thuringiensis (Bt) Cry1F protein is an insecticidal crystalline protein expressed by the Cry1F gene in certain strains of genetically modified plants. This detection is suitable for the qualitative detection of Bt Cry1F in corn seed, cotton seed, leaf tissue, fresh water, and soil samples (please refer to the appropriate sample preparation or extraction). If necessary, positive samples can be confirmed by PCR or other conventional methods.

The Bt Cry1F ELISA GMO Detection Kit should to be stored in the refrigerator (4–8°C). The solutions must be allowed to reach room temperature (20-25°C) before use. Reagents may be used until the expiration date on the box.

The positive control solution in the test kit contain small amounts of Bt Cry1F. In addition, the substrate solution contains tetramethylbenzidine (TMB) and the stop solution contains diluted sulfuric acid. Avoid contact of sub-strate and stopping solutions with skin and mucous membranes. If these reagents come in contact with the skin, wash with water.

Bt Cry1F, a protein derived from the bacteria Bacillus thuringiensis, is expressed in certain genetically modified (GM) corn and cotton plants, such as Herculex® corn and WideStrike® cotton. The Cry1F protein targets the larvae of multiple Lepidopteran (moth) species by binding to specific receptors in the larva’s gut with cascading effects that ultimately leads to death (1).
Routes of exposure affecting regulatory decisions include direct contact, such as non-target organisms feeding on crop tissues, seeds, or plant residues containing Cry1F, and indirect contact, such as exposure to the toxin in pollen, soil contaminated by decomposing plant material containing Cry1F, and predators consuming target species (2).
Although initial testing showed low enviromental impact and toxicity, controversy has arisen over the long-term impact of GM crops on the environment and whether or not GM foods are safe for consumption. Several animal studies have shown serious health risks, such as infertility, immune problems, accelerated aging, faulty insulin regulation, and changes in major organs including the gastrointestinal system, can be associated with the consumption GM products (3).
Bt proteins have been shown to leach into the soil and streams (4).
Efforts like the Non-GMO Project are raising awareness towards the growing number of problems associated with this technology and generat-ing a larger public demand for manufacturers to label their products accordingly.
The Bt Cry1F ELISA GMO Detection Kit allows for the analysis of 46 samples in duplicate determination. The test can be performed in 90 minutes.

ELISA Detection

Corn seed, cotton seed, leaf tissue, surface water, and soil samples