Product Name
Anti-RbcL, Rubisco large subunit, form I Antibody
Immunogen
KLH-conjugated synthetic peptide derived from all known plant, algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana, UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1.
Purity
Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Storage
Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Recommended Dilution
(IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 µg of total cellular protein, (WB)
Tested Applications
Immunogluorescence (IF), Immunolocalization (IL), ImmunoGold (IG), Western blot (WB)
Estimated MW | Detected MW
52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
Predicted Reactivity
Algae, Dicots, Conifers, Liverworts, Mosses, Prochlorophytes, Welwitschia.
Not Reactive
No confirmed exceptions from predicted reactivity are currently known.
Datasheet 