Product Name
Anti-ACO1, Aconitase Antibody
Immunogen
Arabidopsis ACO1 (AT4G35830, Q42560), codon 120 – 898 (C-terminus), was cloned in fusion with a N-terminal 6xHis tag, and over-expressed in E. coli. All recombinant protein accumulated in inclusion bodies, which were purified by centrifugation and solubilised in 6 M guanidine-HCl. The protein was refolded by dilution in 100 mM Tris-HCl 8.5, 10% (v/v) glycerol, 2 mM dithiothreitol, and concentrated prior to immunisation.
Reconstitution
For reconstitution add 100 µl of sterile water.
Recommended Dilution
1 : 5 000 -1 : 10 000 (WB), At higher concentrations the antibody binds aspecifically resulting in non-specific signals around 60 kDa, including Rubisco subunits
Tested Applications
Western blot (WB)
Estimated MW | Detected MW
98 kDa, Note that ACO1, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE,
Predicted Reactivity
Cucurbita maxima, Hordeum vulgare, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Vitis vinifera, Oryza sativa, Zea mays, Picea sitcHensis, Populus trichocarpa.
Not Reactive
Chlamydomonas reinhardii, cyanobacteria
Datasheet 