Product Name
Bt-Cry3A ELISA GMO Detection Kit (480)
Product Overview
Bt endotoxins are proteins produced in plants or in culture expressed by genes taken from the bacterium, Bacillus thuringiensis. Using this test system, you can detect Bt endotoxin extracted from transgenic potato sprouts, tubers and leaves expressing the truncated Cry3A gene.
Storage
Store all kit components at the recommended temperature (above) to assure their full shelf life. Each ELISA plate pouch contains a desiccant packet. Keep the plate or unused testwells sealed in the pouch with the desiccant and store in the refrigerator (2 - 8 °C) between uses. Allow the components of the kit to warm to room temperature for about 30 minutes before using.
Warning
Prevent direct skin and eye contact with, or ingestion of, product components. Obtain medical attention in case of accidental ingestion of kit components. Always wash hands thoroughly after using this product.
Kit Components
Antibody-coated 96-well microtiter plates;
Alkaline phosphatase enzyme conjugate;
PNP substrate tablets, 5 mg each;
PNP substrate buffer, 5X concentrate;
Positive control;
Nonfat dried milk;
PBST buffer, 20X concentrated liquid;
Tween-20.
Materials Required but Not Supplied
Distilled or purified water;
Paper towels;
Micropipette;
Micropipette tips;
Lyophilized negative control;
Sample grinding device;
Airtight container for incubations.
Scientific Background
The Bt-Cry3A ELISA GMO Detection Kit (480) was developed for the detection of or the absence of Cry3A protein expressed from NewLeaf® transgenic potato events. Bt endotoxins are proteins produced in plants or in culture expressed by genes taken from the bacterium, Bacillus thuringiensis. Using this test system, you can detect Bt endotoxin extracted from transgenic potato sprouts, tubers and leaves expressing the truncated Cry3A gene.
Detection method
ELISA Detection
Sample Type
Potato sprouts, tubers and leaves
Datasheet 
