Product Name
Ammonia Colorimetric Assay Kit II
Product Overview
This kit provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In this non-enzymatic assay, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (OD670 nm) using a plate reader. The kit can detect less than 1 nmol (~10 μM) ammonia using either a 96 well or 384 well microwell plate.
Description
Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance.
Applications
The kit can detect less than 1 nmol (~10 μM) ammonia using either a 96 well or 384 well microwell plate.
Kit Components
• Ammonia Reagent 1
• Ammonia Reagent 2
• Ammonium Chloride Standard (100 mM)
Detection method
Absorbance (670 nm)