Ascorbic Acid Colorimetric/Fluorometric Assay Kit

AgK-308

Ascorbic Acid Colorimetric/Fluorometric Assay Kit

Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples. In this assay, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.

Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples.

The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.

Store at -20°C.

100 assays

• Ascorbic Acid Assay Buffer
• Ascorbic Acid Probe (in DMSO)
• Catalyst
• Ascorbic Acid Enzyme Mix (lyophilized)
• Ascorbic Acid Standard (20 µmole)

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)