Cholesterol Detection Kit (cell-based)

AgK-316

Cholesterol Detection Kit (cell-based)

Cholesterol Detection Kit provides a simple, easy to perform, histochemical method of identification of unesterified cholesterol.

Cholesterol is an essential structural component of animal cell membranes. It is required to maintain membrane structural integrity and fluidity. Cholesterol also serves as a precursor for the biosynthesis of several hormones, bile acid and vitamin D. Plasma membrane contains majority of the cellular cholesterol (80-90%) whereas little cholesterol resides in endoplasmic reticulum and in mitochondrial membrane. Transport of intracellular cholesterol within the cells to different compartments is through vesicular and non-vesicular pathways. Defects in these transport processes can alter cellular cholesterol metabolism resulting in pathological conditions. Filipin III is widely used as a probe for sterol localization in membranes. Interaction with cholesterol alters Filipin absorption and fluorescence spectra.

Screen/study/characterize stimulators/inhibitors that affect cholesterol transport and localization in a variety of cells.

Store at -20°C.

100 assays

• Fixative Solution
• Assay Buffer
• Staining Dye

6-well, 12-well, 24-well or 96-well plate
100% Ethanol
37°C Incubator with 5% CO2
Light and fluorescence microscope with UV filter set Ex/Em = 340-380/385-470 nm.

Light and Fluorescence Microscopy (Ex/Em = 340-380/385-470 nm).

Cells