Product Name
Free Fatty Acid Quantification Colorimetric/Fluorometric Kit
Product Overview
Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric
Description
Fatty Acids play very important roles in normal metabolism and many disease developments. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. (spectrophotometry at λ = 570 nm) or fluorometric (at Ex/Em = 535/587 nm) methods with detection limit 2 µM free fatty acid in variety samples.
Applications
The kit has a detection limit 2 μM free fatty acid in variety samples.
Kit Components
• Fatty Acid Assay Buffer
• Fatty Acid Probe (in DMSO)
• ACS Reagent
• Enzyme Mix
• Enhancer
• Palmitic Acid Standard (1nmol/µl)
Detection method
Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Datasheet 
