Galactose and Lactose Colorimetric/Fluorometric Assay Kit

AgK-342

Galactose and Lactose Colorimetric/Fluorometric Assay Kit

Galactose / Lactose Assay Kit provides a simple, convenient means for direct measurement of galactose and lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). To detect Galactose, galactose oxidase specifically oxidizes free galactose, generating a product that reacts with the Galactose Probe to produce color (O.D 570nm) and fluorescence (Ex/Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to generate free galactose which is then measured. Thus, Lactose level = Total Galactose – Free Galactose.

Galactose (C₆H₁₂O₆ FW: 180.16) and lactose (C₁₂H₂₂O₁₁ FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.

N/A

Store at -20°C.

100 assays

• Galactose Assay Buffer
• Galactose Probe (DMSO solution)
• Lactase (Lyophilized)
• Galactose Enzyme Mix (Lyophilized)
• HRP (Lyophilized)
• Galactose Standard (100nmol/µl)

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)