Glucose and Sucrose Colorimetric/Fluorometric Assay Kit

AgK-345

Glucose and Sucrose Colorimetric/Fluorometric Assay Kit

Glucose and Sucrose Assay Kit provides a convenient means for measuring glucose and sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). To measure glucose level, glucose oxidase specifically oxidizes free-glucose generating a compound that reacts with the glucose probe to produce resorufin, which can be detected colorimetrically (O.D. 570 nm) or fluorometrically (Ex/Em 535/587). To measure sucrose, invertase can be added to the reaction to convert sucrose to free glucose and fructose, so total glucose level can be measured. Then the sucrose level = Total Glucose-Free Glucose.

Glucose (C₆H₁₂O₆; FW: 180.16) and sucrose (C₁₂H₂₂O₁₁; FW:342.3) are the important fuel sources to generate universal energy molecule ATP. Measurement of glucose or sucrose level can be very important in both research and development process. Sucrose is a disaccharide which can be converted into one glucose and one fructose when adding Invertase.

N/A

Store at -20°C.

100 assays

• Glucose Assay Buffer
• Glucose Probe (in DMSO)
• Invertase (Lyophilized)
• Glucose Enzyme Mix (Lyophilized)
• Sucrose Standard (100 nmol/l)

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)