Product Name
Magnesium Colorimetric Assay Kit
Product Overview
Magnesium Assay Kit provides a simple sensitive means of quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg²ᶧ. An enzyme linked reaction leads to formation of an intensely colored (λmax = 450nm) product whose formation is proportional to Mg²ᶧ concentration. The linear range of the assay is 2-15 nmoles with detection sensitivity~ 40 µM.
Description
Magnesium is the 11th most abundant element by mass in the human body. Mg²ᶧ is essential to all Living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg²ᶧ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg²ᶧ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.
Applications
The linear range of the assay is 2-15 nmoles with detection sensitivity~ 40 μM.
Kit Components
• Magnesium Assay Buffer
• Magnesium Developer
• Magnesium Enzyme Mix
• Magnesium Standard (150 nmol/µl)
Detection method
Absorbance (450 nm)
Datasheet 
