Product Name
Methanol Assay Kit (Colorimetric)
Product Overview
Methanol Assay Kit utilizes an enzymatic mechanism by which conversion of methanol is correlated stoichiometrically with generation of a colorimetric signal that can be quantified at 450 nm. The assay shows greater than 100-fold specificity for methanol over ethanol. The method is suitable for use in a range of biological and consumable samples, and can detect as little as 500 pmol methanol.
Description
Methanol is the simplest alcohol, consisting exclusively of a methyl group and a hydroxyl moiety. It is both an important industrial molecule (solvent, fuel, building block for chemical synthesis) and a biological metabolite; many bacteria generate methanol as a result of anaerobic metabolism.
Applications
Determination of methanol in biological samples, anaerobic bacterial cultures, agricultural products and food products
Kit Components
• Methanol Assay Buffer
• Methanol Developer
• Methanol Enzyme Mix
• Methanol Probe
• Pure Methanol Stock (24.7 M)
Materials Required but Not Supplied
Plate Reader capable of 37°C setting and absorbance readings
96-well clear plates
Detection method
Absorbance (450 nm)
Compatible Sample Types
Serum, Plasma, Urine; Fruit juice, other non-alcoholic beverages; Microbial Cultures (anaerobic)
Datasheet 
