Phospholipid Assay Kit (Colorimetric/Fluorometric)

AgK-377

Phospholipid Assay Kit (Colorimetric/Fluorometric)

This kit offers an excellent and efficient method for high-throughput assay that can detect phospholipid in the range of 0.1 to 10 nmol per sample

Phospholipids are a major component of lipid bilayer membranes. In addition, phospholipids are biologically important molecules as they are closely involved in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline moiety. In human serum and plasma, almost 95% of the phospholipids are choline-containing phospholipids such as phosphatidylcholine and sphingomyelin. The remaining 5% is mainly choline-free phospholipids (i.e. phosphatidylethanolamine and phosphatidylserine). Phospholipid concentration is considered a disease biomarker as it can be altered in many lung, liver and heart diseases. phospholipid assay kit is simple, accurate and reproducible to measure total choline-containing phospholipids in biological samples such as serum, plasma and exosomes. The signal is directly proportional to the phospholipid concentration of phospholipids in the sample.

Measures phospholipids in the range of 0.1 to 10 nmol per sample

Store at -20°C.

100 assays

• Assay Buffer
• Probe Solution
• Hydrolysis Enzyme
• Development Mix
• Standard (10 µmol)

96-well clear flat-bottom or black fluorometric flat-bottom plate
Multi-well spectrophotometer

Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Serum, plasma and exosomes