Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

AgK-406

Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based) enables detection and monitoring of autophagy and autophagic cell death in cultured cells. The kit contains two fluorescent probes: a membrane permeable selective autophagy stain and a cell death marker. An autophagy-inducing positive control reagent, which increases autophagy staining and serves as an experimental control. This easy-to-use non-radioactive kit allows researchers to study the regulation of autophagy and cytotoxicity at the cellular level by Fluorescence Microscopy and Flow Cytometry in cultured cells.

Autophagy or autophagocytosis (“self-eating”) refers to a process of degradation of cytoplasmic components within lysosomes, a unique process mediated by autophagosomes. Autophagy consists of several sequential steps: sequestration, transport to lysosomes, degradation and eventual re-utilization of degradation products. Autophagosomes engulf a portion of cytoplasm, thus autophagy is generally thought to be a nonselective degradation system regulated by many different cellular signaling pathways. Autophagy functions as a stress response upregulated by nutrient and energy starvation, oxidative stress, or other harmful conditions (such as damage to organelles, protein aggregation and infection by pathogens). Dysfunction of autophagy is associated with many human cancers and neurodegenerative diseases.

Measurement of Autophagy processes
Screening compounds affecting Autophagy

Store at -20°C.

50 assays

• Assay Buffer (10X)
• Autophagy Stain (500X)
• Cell Death Marker (500X)
• Positive Control Reagent (10000X)

96-well clear plate with flat bottom
Multi-well spectrophotometer

Flow Cytometry (FL1/FL2)

Serum, plasma, blood, urine & other body fluids