DL-Serine Assay Kit (Fluorometric)

AgK-410

DL-Serine Assay Kit (Fluorometric)

DL-Serine Assay Kit allows for quantification of both L- and D-Serine in biological fluids and tissues. The assay is based on the conversion of L-Serine to D-Serine, which is metabolized to an intermediate product that is subsequently oxidized and reacts with a probe to form a stable fluorophore (Ex/Em = 539/587 nm). Samples may be divided and assayed simultaneously for quantification of both D-Serine and total DL-Serine. The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect less than 1 μM Serine.

Serine is one of the 20 naturally-occurring amino acids used by all organisms in the biosynthesis of proteins. Having a single chiral center, serine can exist as either of two stereoisomers (L-Serine and D-Serine). In addition to its role as a protein building block, L-Serine serves as a precursor to many vital biomolecules, including phosphatidylserine, sphingomyelin and the amino acids glycine and cysteine. The non-proteogenic isomer D-Serine is also synthesized from L-Serine in the mammalian brain. While most D-amino acids are only present in trace amounts in mammals, D-Serine is a vital neurotransmitter that acts as a co-agonist of synaptic N-methyl-D-aspartate (NMDA) type glutamate-gated ion channels. Impaired D-Serine metabolism may contribute to several neuropsychiatric disorders, including depression, schizophrenia, epilepsy and dementia.

Estimation of Serine in various biological samples

Store at -20°C.

100 assays

• Serine Assay Buffer
• Probe Solution
• Serine Racemase Enzyme Mix
• D-Serine Enzyme Mix
• Developer Enzyme Mix
• Sample Cleanup Mix
• D-Serine Standard

Multiwell fluorescence microplate reader
Black 96-well plates with flat bottom
10 kDa Spin Column

Fluorescence (Ex/Em 535/587 nm)

Human or animal biological fluids (plasma, serum, CSF, etc.)
Soft tissue homogenates (i.e. liver, brain, etc.)
Cultured cell lysates (adherent or suspension cells)