Fructosamine Assay Kit (Colorimetric)

AgK-411

Fructosamine Assay Kit (Colorimetric)

Fructosamine Assay Kit is a microplate-based colorimetric assay for the direct determination of fructosamine levels in serum. The assay is based on the ability of fructosamine to reduce nitroblue tetrazolium (NBT), forming a colored end-product (purple) under alkaline conditions. The formation rate of formazan is proportional to the concentration of fructosamine in samples and the increase in absorbance (OD 530nm) can be monitored using a spectrophotometer. The kit includes a Thiol Blocking Reagent and a Sample Cleaning Mix that minimizes the interference of other endogenous reducing agents which ensures accurate measurements of Fructosamine in biological samples. The assay is simple, reproducible and can detect as low as 20 µmol/L of fructosamine in samples.

Fructosamines are stable glycated proteins that are formed by a non-enzymatic reaction between glucose and serum proteins (usually albumin). Elevated concentrations of fructosamine can be found in serum samples of diabetic patients and its detection can be used to assess the glycemic status of diabetics. The half-life of albumin is shorter when compared to hemoglobin (t1/2= 20 days vs. 50 days respectively). Thus, fructosamine levels reflect the efficacy of treatment in diabetic patients during short-term periods, and provide earlier and more sensitive detection for diabetes than many other carbohydrate tests.

Measurement of Fructosamine levels in serum

Store at -20°C.

100 assays

• Fructosamine Buffer A
• NBT (in DMF)
• Thiol Blocking Reagent
• Sample Cleaning Mix (Lyophilized)
• Fructosamine Buffer B
• Fructosamine Calibrator (3.2 mM)

96-well clear plate with flat bottom
Multi-well spectrophotometer

Absorbance (OD 530 nm)

Serum