Product Name
Glycerophosphorylcholine Assay Kit (Colorimetric/Fluorometric)
Product Overview
Glycerophosphorylcholine Assay Kit allows a quick and sensitive means to determine GPC concentration in a biological sample. The assay is robust and specific, with a GPC enzyme mix converting GPC in the sample into an intermediate that is then utilized to quantitatively generate signal using the GPC Developer mix and GPC Probe. The included Sample Clean-Up Mix allows the user to avoid high background from choline, which is present at high levels in many biological fluids. The assay is rapid, simple, high throughput compatible, and can detect GPC concentrations as low as 0.1 µM in tissue lysates and other samples such as breast milk and seminal plasma.
Description
Glycerophosphorylcholine (GPC), also referred to as alpha-GPC or choline alfoscerate, is a small metabolite and potential dementia treatment. GPC is a breakdown product of phosphatidylcholine, and dysregulation of phospholipid metabolism can alter lipid and metabolite concentrations, leading to health problems such as fatty liver, Gaucher’s disease, and Tay-Sachs disease. GPC is also component of seminal fluid and breast milk, and a precursor of the neurotransmitter, acetylcholine. As GPC can efficiently cross the blood-brain barrier, it has been hypothesized as a treatment for Alzheimer’s disease and other forms of dementia. In such cases, GPC serves as a biomarker for lipid membrane decomposition.
Applications
• Measurement of GPC content of various tissues/cell extracts
• Determination of GPC concentration in biological fluids
Kit Components
• GPC Assay Buffer
• GPC Enzyme Mix
• GPC Developer
• GPC Probe (in DMSO)
• Sample Clean-Up Mix
• GPC Standard (1 µmole)
Materials Required but Not Supplied
Clear or Black (fluorometric only) 96-well plate with flat bottom
Multi-well spectrophotometer
10 kDa Spin Column
Deproteinizing Sample Preparation Kit
Detection method
Absorbance (570 nm) or Fluorescence (Ex/Em= 535/587 nm)
Compatible Sample Types
Tissues (e.g. liver, lung)
Biological fluids (e.g. seminal plasma, breast milk)
Datasheet 
